I am a biomedical researcher.
I study the functional and molecular implications of cancer-associated mutations in a critical tumor suppressor. I use biochemical and molecular biology approaches to investigate the molecular landscape of common hotspot mutations seen in endometrial cancers.
- (Conference proceeding) Kafle CM, Bell DW. Investigation of the mechanism of PADI2 regulation by the FBXW7 tumor suppressor. Clinical Cancer Research, 2024. DOI
Prior to this, I studied Adenovirus-induced cellular DNA damage response, cell cycle, and DNA replication. The findings from my work show that Ad-induction of DDR is necessary for the activation of G2/M checkpoint kinases in E4-mutant infected cells. We also showed that E4-mutant infected cells are arrested at a phase characterized by either 4N or >4N DNA content while preventing cells from going through mitosis.
- Kafle CM, Anderson AY, Prakash A, Swedik S, Bridge E. An Adenovirus early region 4 deletion mutant induces G2/M arrest via ATM activation and reduces mitotic marker expression. Virology, 2021. PMID: 34626906
I also investigated the efficacy of artemisinin–lumefantrine–based combination therapy (ACT) for the treatment of Plasmodium falciparum malaria in Nepal. In this multi-institutional collaborative study, we conducted a therapeutic efficacy assessment among falciparum malaria patients treated with ACT in regions of Nepal characterized by high mobility of migrant workers.
- Ghimire P, Rijal KR, Kafle CM, et al. Efficacy of artemether-lumefantrine for treating Plasmodium falciparum malaria in Nepal. Tropical Diseases, Travel Medicine and Vaccines, 2018. PMID: 30123520
- Kafle CM, Ghimire S, Dhakal P, Shrestha U. Identification of parasites in soil samples of vegetable fields in Bhaktapur District. Tribhuvan University Journal of Microbiology, 2014.
I occasionally write for local publications and take photographs during my hikes.