My research project investigates the impact of Adenovirus (Ad) infections on the host cell division cycle (cell cycle), a series of highly regulated events comprising DNA replication and cell division. Under the mentorship of Dr. Eileen Bridge, my research has demonstrated that Ad infection induces cellular DNA replication while preventing cell division. Ad E4 proteins are known for their roles in regulating the cellular DNA damage response, and our study shows that an Ad E4-mutant arrests the host cell cycle by activating G2/M checkpoint kinases through the kinases involved in the DNA damage response pathway. We are currently investigating mechanisms of the shutdown of host cell DNA replication at late times of Ad infection.
For the fulfillment of my master's degree, I studied the efficacy of artemether-lumefantrine for the treatment of uncomplicated Plasmodium falciparum malaria in Nepal. We found that the combination therapy using artemether-lumefantrine was effective for the treatment of uncomplicated falciparum malaria in Nepal. This project was advised by Dr. Prakash Ghimire and Dr. Komal Raj Rijal.
- An Adenovirus early region 4 deletion mutant induces G2/M arrest via ATM activation and reduces expression of the mitotic marker phosphorylated (ser10) histone 3. Virology. 2021 Sep 27;565:1-12. doi: 10.1016/j.virol.2021.09.006. [Epub ahead of print] PubMed PMID: 34626906.
- Efficacy of artemether-lumefantrine for the treatment of uncomplicated Plasmodium falciparum malaria in Nepal. Trop Dis Travel Med Vaccines. 2018;4:9. doi: 10.1186/s40794-018-0068-2. eCollection 2018. PubMed PMID: 30123520; PubMed Central PMCID: PMC6092778.
- Fall 2020